Development and Characterization of EST-SSR Markers in Stipa breviflora (Poaceae)
نویسندگان
چکیده
منابع مشابه
Development and characterization of EST-SSR markers in Stipa breviflora (Poaceae)1
PREMISE OF THE STUDY Stipa breviflora (Poaceae) is one of the dominant species of the desert steppe in the eastern Eurasian grasslands. Simple sequence repeat (SSR) markers were developed for use in genetic diversity studies of this species. METHODS AND RESULTS A total of 1954 potentially polymorphic loci were obtained by comparing transcriptome data of eight different S. breviflora individua...
متن کاملDevelopment and characterization of 29 polymorphic EST-SSR markers for Stipa purpurea (Poaceae)1
PREMISE OF THE STUDY Expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed using Illumina sequencing for further genetic diversity studies of Stipa purpurea (Poaceae). METHODS AND RESULTS Twenty-nine polymorphic and eight monomorphic EST-SSR loci were developed and characterized in 90 individuals from nine S. purpurea populations. The number of alleles per locus range...
متن کاملDevelopment and Characterization of Polymorphic EST-SSR and Genomic SSR Markers for Tibetan Annual Wild Barley
Tibetan annual wild barley is rich in genetic variation. This study was aimed at the exploitation of new SSRs for the genetic diversity and phylogenetic analysis of wild barley by data mining. We developed 49 novel EST-SSRs and confirmed 20 genomic SSRs for 80 Tibetan annual wild barley and 16 cultivated barley accessions. A total of 213 alleles were generated from 69 loci with an average of 3....
متن کاملCharacterization and development of EST-SSR markers in sweet potato (Ipomoea batatas (L.) Lam)
In this study, a cDNA library was constructed from the total RNA of sweet potato leaves. A total of 789 copies of the cDNA were cloned in Escherichia coli by employing the pGEM-T Easy vector. Sequencing was carried out by Solgent Co. (Korea). As many as 579 expressed sequence tag-simple sequence repeat (EST-SSR) markers were designed (73.38%) from the known cDNA nucleotide base sequences. The l...
متن کاملDevelopment and characterization of EST-SSR markers in Ostryopsis (Betulaceae)1
UNLABELLED PREMISE OF THE STUDY A set of expressed sequence tag (EST) microsatellite markers were developed and characterized using next-generation sequencing technology for the Chinese genus Ostryopsis (Betulaceae). • METHODS AND RESULTS A total of 38 high-quality simple sequence repeat (SSR) primers were identified, of which 15 could be successfully amplified. Subsequently, we selected 8...
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ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2017
ISSN: 2168-0450
DOI: 10.3732/apps.1600157